Characterization of adipose depot-specific stromal cell populations by single-cell mass cytometry

Characterization of adipose depot-specific stromal cell populations by single-cell mass cytometry

Blog Article

Summary: The increased prevalence of obesity and metabolic diseases 1964-1973 Fuel System has heightened interest in adipose tissue biology and its potential as a therapeutic target.To better understand cellular heterogeneity and complexity of white adipose tissue (WAT), we employed cytometry by time-of-flight (CyTOF) to characterize immune and stromal cells in visceral and subcutaneous WAT depots under normal and high-fat diet feeding, by quantifying the expression levels of 32 surface marker proteins.We observed comparable proportions of immune cells in two WAT depots under steady state, but depot-distinct subtypes of adipose precursor cells (APC), suggesting differences in their adipogenic and fibrogenic potential.Furthermore, in addition to pro-inflammatory immune cell shifts, significant pro-fibrotic changes were observed in APCs under high-fat diet, suggesting that APCs are early responders to dietary challenges.

We propose CyTOF as a complementary and alternative tool to current high-throughput Meters single-cell transcriptomic analyses to better understand the function and plasticity of adipose tissue.